EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.22.2 | biotechnology | papain immobilization on magnetic composite microspheres at pH 8.0 and 30°C, quantification and kinetics, overview, the immobilized enzyme exhibits better environmentally adaptability and reusability than the soluble enzyme | Carica papaya |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.2 | additional information | - |
additional information | kinetics and thermodynamics of soluble and immobilized enzyme at pH 7.0 and 40°C, overview | Carica papaya |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.2 | Carica papaya | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.2 | commercial preparation | - |
Carica papaya | - |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.22.2 | 4°C, 60 days, the immobilized enzyme shows 20% loss of activity, the soluble enzyme shows 50% loss of activity | Carica papaya |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.2 | casein + H2O | - |
Carica papaya | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.2 | 65 | - |
soluble enzyme | Carica papaya |
3.4.22.2 | 80 | - |
immobilized enzyme | Carica papaya |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.2 | 20 | 90 | temperature profile of soluble and immobilized enzyme | Carica papaya |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.2 | 70 | - |
30 min, the immobilized enzyme is completely stable, while the soluble enzyme loses activity | Carica papaya |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.2 | 6.5 | - |
soluble enzyme | Carica papaya |
3.4.22.2 | 8 | - |
immobilized enzyme | Carica papaya |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.2 | 5 | 10 | pH profile of soluble and immobilized enzyme | Carica papaya |